In which blood collection method is anesthesia typically not required and multiple samples can be collected in a single day?

The saphenous vein collection method is often used because it typically allows for the collection of blood samples without requiring anesthesia. This is particularly beneficial in laboratory settings where multiple blood samples from the same animal may be necessary throughout the day. The saphenous vein is located in the leg, and accessing it is relatively straightforward and less invasive compared to other methods.

Using this technique, a technician can obtain adequate blood volume while minimizing stress or discomfort to the animal, thereby enabling them to undergo repeated sampling within a short timeframe, such as within the same day. This characteristic is essential in research settings where monitoring physiological changes after treatment or intervention is vital.

In contrast, other methods such as retro-orbital bleeding typically require anesthesia because of the associated discomfort and potential trauma to the animal, while cardiac puncture is more invasive and generally not performed without anesthesia due to its nature and risks involved. Intraarterial blood collection also tends to be more invasive and usually necessitates sedation or anesthesia, as it involves accessing arteries, which carries a higher risk of complications.