What does a copulatory plug indicate in breeding observations?

A copulatory plug is a biological indicator left in the female's reproductive tract after mating has occurred, typically composed of ejaculate and vaginal secretions. When observed during breeding observations, the presence of a copulatory plug confirms that mating has successfully taken place between a male and female animal. This plug serves as a physiological response to prevent further mating and protect the sperm, potentially increasing the chances of successful fertilization.

Confirmation of pregnancy relies on additional factors beyond just the presence of a copulatory plug. These might include hormonal changes, physical signs in the female, or eventually the detection of embryos through methods such as ultrasonography. Similarly, a successful birth is indicated by the arrival of offspring rather than the plug itself, and a genetic anomaly pertains to the characteristics of the offspring and not to the breeding event. Hence, the correct interpretation of a copulatory plug as a sign that mating has occurred aligns directly with its biological function and purpose during reproduction.